Free glucose is formed during glycogenolysis fromA. glucose residues in 1, 4 linkage to the main chainB. glucose residues in 1, 6 linkage to the main chainC. glucose 1,6 - diphosphate hydrolysisD. glucose 1 - phosphate hydrolysis A common featu of all serine proteases isA. autocatalytic activation of zymogen precursorB. presence of Ser - His -Asp catalytic triad at the active site .C. tight binding of pancreatic trypsin inhibitorD. cleavage of protein on the carboxyl site of serine residues Aspartic protease P. possesses two aspartic acid residues at the active site. Q. includes enzymes like pepsin, cathepsin and renin R. involves formation of intermediate amide dihydrate. S. present in AIDS virus HIV-1A. P and SB. P, Q and SC. P and QD. P, Q, R and S Which of the following vitamins does not act as a precursor for coenzymes?A. Vitamin B2B. BiotinC. Ascorbic acidD. Folic acid In enzyme kinetics Vmax reflectsA. Enzyme substrate complexB. Amount of an active enzymeC. Substrate concentrationD. Half the substrate concentration A demonstrable inducer is absent inA. Inhibited enzymeB. Allosteric enzymeC. Constitutive enzymeD. Co-operative enzyme Glycerol is added to protein solutions to stabilize the preparations byA. preferential hydration of proteinsB. interacting and neutralising the surface charges on the proteinsC. increasing the viscosity of solutionD. stabilizing the pH From the following statements(A) Enzymes enhance reaction rates by a factor of 2 to 10. (B) The activation energy for a reaction is lowered by enzymes. (C) The interactions between enzymes and substrates are hydrogen bonding, hydrophobic and ionic.(D) Substrate concentration does not affect the rate of enzyme catalyzed reactions.Find the incorrect combination.A. A, BB. B, CC. A, CD. A, D Protein conformational dynamics can be determined by which one of the following techniques-Circular DichroismFluorescence spectroscopyDifferential scanning calorimetryFibre diffraction Holiday junction is observed duringA. InterphaseB. RecombinationC. Dna repairD. Telophase of mitosis Which of the following is not an inhibitor of DNA polymerase?A. CytarabineB. AphidicolinC. AmikhellineD. Nalidixic acid Rhizobial genes that participate in legume nodule formation are called nodulation (nod) genes. The nodD-encoded protein.influences the host specificity of Rhizobium.binds to the nod box and induces transcription of all nod genesis an acetyl transferase that adds a fatty acyl chain to the Nod factor.catalyzes the linkage of N-acetyl glucosamine residues Since the pK values for aspartic acid are 2.0, 3.9 and 10.0, it follows that the isoelectric (pH) isA. 6.0B. 3.0C. 5.9D. 3.9 Which one of the following statements regarding exonuclease/ restriction enzymes used in recombinant DNA technology is INCORRECT?A. S1 nuclease acts on double-stranded DNA or RNA termini with nick.B. Type II class can require ATP for its mechanismC. It remove nucleotides, one at a time, from the ends of a sequence.D. Type II class do not recognize palindromic sequences. The MALDI mass spectrum of a peptide gave a single peak with M/z of 1000. The ESI mass spectrum of the same peptide gave single peaks. These observations indicate that:A. The protein sample is monomeric in nature.B. The purity of sample is 100%C. The protein sample contains the single charged species.D. The ionization principle of both ESI and MALDI must be same Which one of the methods listed below is the most sensitive label-added quantification method for proteinsA. UV spectroscopyB. Infra-red spectroscopyC. Raman spectroscopyD. 13C content of protein Actinomycin inhibits which polymeraseA. Dna dependent dna polymeraseB. Dna dependent rna polymeraseC. Rna dependent dna polymeraseD. Rna dependent rna polymerase Which of the following is not an inhibitor of topoisomerase?A. Etoposide - -B. CamptothecinC. DoxorubicinD. Cisplatin Function of Pie 1 is toA. Suppress the establishment of somatic cells fate and preserve the totipitency of germ cell lineageB. Supports Pal 1C. Suppress the establishment of germ cellsD. Encourage the establishment of somatic cell fate Absence of lin 39 function causeA. More than one central cellB. Specification is absentC. Normal vulva formationD. No primary VPC Time is Up!