Morphology and Ultrastructure of Rickettsiae
Rickettsiae are placed into the order Rickettsiales that contains the family Rickettsiaceae.
Rickettsiae pertain to obligate intracellular parasites. They live and multiply within the cytoplasm or nuclei of cells from different tissue types.
Rickettsiae are pleomorphic organisms. Coccoid forms look like tiny single-grain ovoids with the diameter about 0.5 μm; quite often they produce diploforms.
Rod-like rickettsiae make short rods of 1 to 1.5 μm in length with granules on the ends, or they render longer thin curved rods of 3-4 μm.
Filamentous forms are present as the long threads in the range of 10-50 μm in length; in some cases they might be curved.
Rickettsiae have no spores, capsules or flagella.
Electron microscopy revealed that rickettsiae possess inner and an outer membranes with elements of peptidoglycan. Their cytoplasm contains bacterial ribosomes.
Rickettsiae propagate by division of rod-shaped and coccoid forms, and by fragmentation of the filamentous forms; the latter creates daughter rod-shaped and coccoid cells.
For growth and reproduction rickettsiae are cultured within living cells – laboratory cell lines, or embrionated chicken eggs within yolk sacs. Zoonotic rickettsia can be cultured in laboratory animals, e.g. guinea pigs. The bacteria are stained with aniline dyes; for instance, by Romanowsky-Giemsa stain.
There are several specially devised staining methods for examination of rickettsiae. Zdrodovsky method with carbol fuchsin (as modified Ziehl-Neelsen stain) makes rickettsiae red on the blue background of cellular cytoplasm and nuclei. Similar to this technique is Gimenez stain that renders rickettsiae red by fuchsin inside blue-green cellular background made by malachite green.
The vast number of rickettsia can cause the ailments in humans. Severe anthroponotic disease epidemic typhoid fever is caused by Rickettsia prowazekii, zoonotic endemic typhoid fever – by Rickettsia typhi. They are transmitted by lice or by ticks and fleas, respectively.
The large group of zoonotic infections known as spotted fever rickettsioses includes more than 20 pathogenic agents. Among them are R. rickettsii – the agent of Rocky Mountain spotted fever, R. conorii – the agent of Mediterranian spotted fever, and many others. The representative of one more rickettsial genus Orientia tsutsugamushi causes scrab typhus.