A critical enzyme used directly in the synthesis of dTMP (thymidine) isCarbamoyl phosphateAspartate TranscarbamoylaseDihydroorataseThymidylate synthase In which phase of the cell cycle does DNA replication occur?G0G1SG2 A 10 month old baby boy presents with steatorrhea, recurrent pulmonary infections, GI upset and foul-smelling stool. Which of the following tests is undertaken to confirm your diagnosis?Sweat testBlood glucoseCBCRFLP analysis The enzyme responsible for initiating DNA replication in prokaryotes is:DNA polymerase IDNA polymerase IIIPolymerase betaPrimase The enzyme responsible for continuing DNA replication in prokaryotes, once it is initiated is:DNA polymerase IDNA polymerase IIIpolymerase betapolymerase delta Antibiotics such as Ciprofloxacin and Flouroquinolines work by inhibiting a specific enzyme. This enzyme is normally necessary to relieve torsional strain that is caused by the unwinding of the helix. What is the name of this enzyme?DNA ligaseTopoisomerase (DNA Gyrase)single-stranded binding proteinprimase Which of the following techniques is primarily undertaken to amplify DNA?PCRMicroarraysNorthern BlottingSouthern Blotting In Pyrimidine Synthesis, Eukaryotes can use uracil to feedback inhibit which of the following enzymes?Carbamoyl phosphate synthaseAspartate TranscarbamoylaseDihydroorataseThymidylate synthase All of the following are required for PURINE DE NOVO synthesis EXCEPT:CO2GlycineGlutamineIsoleucine Which of the following, in high concentrations, can overcome PURINE DE NOVO inhibition?Ribose-5-phosphatePRPP5-phosphoribosyl 1-amineGDP A 40-year-old alcoholic comes in with severe pain in his big toe. You decide to administer Allopurinol to inhibit which of the following enzymes:Xanthine OxidaseThymidine kinaseAdenosine DeaminaseAdenine phosphoribosyl transferase All of the following are used in PCR EXCEPT:Taq polymeraseRestriction enzymesOligonucleotide primersDeoxynucleoside triphosphates The following are features of DNA replication EXCEPT:Semi-conservativeSemi-discontinuousunidirectionalchain growth in the 5‟ –> 3‟ direction The oncogene Ras binds:ATPGTPGlucoseHemoglobin Which out of the following mechanisms is involved in the production of variety of immunoglobulins each specific for a specific antigen?Class switchingGene amplificationGene rearrangementRNA editing Which out of the followings is a common enzyme for de novo as well as salvage pathway of purine biosynthesis?AmidotransferasePRPP synthetaseHGPRTaseAdenylosuccinate synthetase Which of the following does not have introns?DNANon-processed pseudo genesProcessed m RNAPrimary RNA transcript Which of the following is NOT true of RNA synthesis?The key enzyme is RNA polymeraseThe energy is supplied by ring cleavageThe RNA sequence is complementary to the template strand of DNAThe RNA sequence is of opposite polarity to the template strand of DNA If the molar amount of G in a DNA sample is 20%, what is the molar amount of T in the sample?20%30%40%60% With respect to the LAC operon, if both glucose and lactose are present and glucose is low, which of the following is NOT true?High CAPincreased uptake of lactoselow cAMPincreased transcription of the lac operon Time is Up!