What is differential interference contrast (DIC) microscopy?

QuestionsCategory: QuestionsWhat is differential interference contrast (DIC) microscopy?
Biology Ease Staff asked 1 year ago

The DIC microscopy like phase contrast microscopy uses differences in refractive indexes. However, a DIC microscope uses two beams of light instead of one. Besides, the prisms split each light beam adding contrasting colors to the specimen. In this way, the resolution of a DIC microscope is higher than that of a phase contrast microscope. The image is brightly colored and is nearly three-dimensional in appearance.