Types of plant tissue culture

Types of plant tissue culture


  • Plant tissue culture is a collection of techniques used to maintain or grow plants cell, tissues, or organs under sterilized conditions on a nutrient culture of known composition.
  • Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation.

Choice of explant

  • The tissue obtained from a plant to be cultured is called an explant and may include a portion of shoots, leaves, stems, flowers, roots, and single undifferentiated cells.
  • The most commonly used tissue explant is the meristematic and ends of the plants like the same keep auxiliary root tip.
  • These tissues have high rates of cells division and either concentrate or produce required growth-regulating substances including auxins and cytokinins.

Tissue culture techniques

Callus culture

  • The callus is a mass of highly vacuolated unorganized self resulting as a consequence of building in plants and in tissues culture with the use of sophisticated techniques.
  • Cell maybe from Poland Anthrax bird fruits leaf shoot Apex etc.
  • Embryoid develops into plantlets and letters in a whole viable plant.


  • Aseptic preparation of plant material.
  • Selection of suitable growth medium
  • Selection of suitable controlled physical growth conditions for incubation.

Types of plant tissue culture
A meristem is a group of undifferentiated plant cells that can undergo division to form all types of tissues.

Meristem culture

  • Generally, explant is used in the shiny dome-shaped structure of length which is less than 10 0.1 mm with one or two pairs of the youngest leaf.
  • Meristem tissue is isolated and activated on a sweet table growth medium under aseptic conditions.
  • Maritime forms callus at adds cut and on which a large number of shoot primordial develop.

Application of meristem culture:

  • Virus and parasite elimination
  • The resulting plantlets are often free of viruses and parasites.
  • Meristem is devoid of viruses those are certain reasons behind this.
  • Meristematic cells are actively dividing and have high metabolic activity.

Organ culture

  • In organ culture-specific organ is excised culture. On a suitable growth nutrient medium under aseptic and controlled physical concentration.
  • The particular organ treat and its characteristics structure and feature and continue to grow as usual unlike in callus culture.
  • It includes another culture pollen culture of embryo, ovary, root, shoot, floral, fruits seed etc.

Suspension culture:

  • Tissue and cell culture in a liquid medium produce a suspension of single-cell and cell of few too many cells these are called suspension culture.
  • Suspension culture grows much faster than callus culture need to be subcultured about every week also allow a more accurate determination of the nutritional requirement of cell and are the only system to scale off for large-scale production of cell and events somatic embryos.
  • The suspension culture is the group like batch culture, continuous culture, and immobilized cell culture.

Pollen and Anthrax culture:

  • Haploid plants may be obtained from pollen grains by placing another or isolated pollen grains on a sweet table culture medium this constituent another and pollen culture respectively.
  • Isolated pollen grains when cultured in vitro give rise to haploid or callus this approach is called pollen culture.

Protoplast culture:

  • The techniques for protoplast culture are pretty well defined and lightly effective for almost all the systems.
  • A number of strategies have been used to include fusion between protoplast of different strains and species.
  • The protoplast mixture is then subjected to high PH and high temperature.
  • This technique is quite suitable for some space ES files for some others it may be toxic.

Types of plant tissue culture