What is differential interference contrast (DIC) microscopy?
The DIC microscopy like phase contrast microscopy uses differences in refractive indexes. However, a DIC microscope uses two beams of light instead of one. Besides it the prisms split each light beam adding contrasting colours to the specimen. In this way resolution of a DIC microscope is higher than that of a phase contrast microscope. The image is brightly coloured and is nearly three-dimensional in appearance.