POLYMERASE CHAIN REACTION-OLIGONUCLEOTIDE LIGATION ASSAY (PCR-OLA)

Genetic mutations lead to the production of defective genes. Such genetic mutation can affect the restriction endonuclease site sometimes but not in all cases....

DNA Fingerprinting and RAPD

DNA Fingerprinting and RAPD DNA FINGERPRINTING “DNA Fingerprinting — The greatest breakthrough in forensic sciences in this century” Identification and characterization of individuals are...

Non-Radioactive Hybridization Methods

NON-RADIOACTIVE HYBRIDIZATION PROCEDURES Nucleic acid hybridization can be detected by labeling the probe with a radioactive isotope or a non-radioactive isotope. Therefore, the hybridization...

DNA Diagnostic

DNA Diagnostic DNA diagnostic means qualitative and quantitative analysis of DNA, RNA, and proteins. The common techniques used are, for DNA — PCR, RFLP...

Nucleic Acid Hybridization

Nucleic Acid Hybridization BASIC CONSIDERATIONS Nucleic acid hybridization is a basic tool in molecular genetics that exploits the ability of single-stranded nucleic acid molecules...

ELISA and Its Types

ELISA and Its Types INTRODUCTION ELISA or EIA is a method widely used for measuring the concentration of a particular molecule (E.g., a hormone...

Cloning Vector (Plasmid)

Plasmid Plasmids are small circular double-stranded, chromosomal DNA molecules, which occur in most bacterial organisms. The term was first introduced by Joshua Lederberg in...

Chromosome banding

Chromosome banding helps chromosome deletions, duplications, translocations, inversions, and other less prominent chromosome anomalies to be defined. Chromosome banding, formed after staining with a...

CRISPR/Cas System in Bacteria

The genome of about 50% of bacterial species and up to 90% of archaebacteria harbors the unique genetic region responsible for so-called “adaptive immune...

Microarrays and its applications

Microarrays and its applications Microarrays are large arrays of DNA sequences attached via automatic equipment to a solid substrate . These displays are also...