Cultivation of viruses
Experiment 1 Aim: Cultivation of viruses in the cell line method. Introduction Viruses are acellular pathogenic organisms that …
Practicals
Microbial assay and its methods
Introduction The microbiological assay is a standard analytical method used to check the potency of antibiotics. It can …
Biochemical Tests
Biochemical Tests Identification of microorganisms is done in two steps; Step I: Examination under the microscope Step II: …
Cellotape Method for Fungus Observation
Aim: Examination of Fungi by cellotape method. Introduction: Fungi are eukaryotic, heterotopic organisms with true nuclei. Fungi are …
Spread plate method: Principle, Procedure, Results
Aim: To perform spread plate method/Lawn method Introduction: The spread plate is a technique used to separate the …
Pour Plate Technique: Principle, Procedure, Results
Aim: To perform pour plate technique Introduction- Pour plate method is most commonly used to quantitate the number …
Streak plate method: Principle, Procedure, Results
Aim: To perform the Streak plate method. Principle: It is a common method used to separate and isolate …
Hanging Drop Technique
Aim: To perform a motility test of a bacteria by hanging drop technique. Introduction: The hanging drop technique …
Giemsa staining
Giemsa staining Aim: To study nuclear material Staining. Introduction: Bacteria are prokaryotic which possess nuclear material and circular …
Flagella Staining: Principle, Procedure and Results
Aim: To study flagella staining. Introduction: The flagellum is a locomotory organ of bacterial cells. Bacterial flagella have …
Metachromatic granule staining
Aim: To study metachromatic granule staining Introduction: Albert’s staining is used to demonstrate metachromatic granules. Albert’s staining describes …
Negative Staining: Principle, Procedure, Results and Application
Aim: To study negative staining of microorganisms. Introduction: Negative Staining is used to study the various shapes, sizes …